Journal: Journal of hepatology

Article Title: IL-17A/F enable cholangiocytes to restrict T cell-driven experimental cholangitis via PD-1/PD-L1 interaction

doi: 10.1016/j.jhep.2020.10.035

Figure Lengend Snippet: TaqMan ® Gene Expression Assay IDs of genes used in this study

Article Snippet: Target gene expression was normalized to Hprt expression and the fold-induction was quantified by normalization to control groups using the ΔΔCt method. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Assay ID Gene Assay ID Ccl2 Mm00441242_m1 IL6 Hs00174131_m1 CCL2 Hs00234140_m1 Il10 Mm00439614_m1 Ccl20 Mm01268754_m1 Il12a Mm00434169_m1 CCL20 Hs00355476_m1 Il23a Mm00518984_m1 Cd274 Mm03048248_m1 Krt19 Mm00492980_m1 CD274 Hs00204257_m1 Ly6c Mm03009946_m1 Col1a1 Mm00801666_g1 Ly6g Mm04934123_m1 Col3a1 Mm01254476_m1 Mmp9 Mm00442991_m1 Cxcl9 Mm00434946_m1 Mmp13 Mm00439491_m1 Cxcl10 Mm00445235_m1 Mpo Mm01298424_m1 Foxp3 Mm00475162_m1 Nfkb1 Mm00476361_m1 Gzmb Mm00442837_m1 Pdcd1 Mm01285676_m1 Hprt Mm03024075_m1 Rorc Mm01261022_m1 HPRT Hs02800695_m1 Tgfb1 Mm01178820_m1 Ifng Mm01168134_m1 Tnfa Mm00443258_m1 Il1b Mm00434228_m1 Vcam1 Mm01320970_m1 Open in a separate window TaqMan ® Gene Expression Assay IDs of genes used in this study

Techniques: Gene Expression, Gene Assay

Journal: Heliyon

Article Title: Xanthohumol alleviates palmitate-induced inflammation and prevents osteoarthritis progression by attenuating mitochondria dysfunction/NLRP3 inflammasome axis

doi: 10.1016/j.heliyon.2023.e21282

Figure Lengend Snippet: Primers for RT-PCR used in this study.

Article Snippet: Primary antibodies specifically directed against GAPDH (1:1000), COX-2 (1:1000), iNOS (1:1000), MMP1 (1:5000), MMP3 (1:5000), MMP13 (1:1000), PGC-1α (1:1000), TFAM (1:1000), AMPKα (1:1000), and p-AMPKα (1:1000) were purchased from Proteintech.

Techniques:

Journal: Heliyon

Article Title: Xanthohumol alleviates palmitate-induced inflammation and prevents osteoarthritis progression by attenuating mitochondria dysfunction/NLRP3 inflammasome axis

doi: 10.1016/j.heliyon.2023.e21282

Figure Lengend Snippet: The cytotoxicity of Xn on human chondrocytes. A. Chemical structure of Xn. B. Human primary chondrocytes examined by toluidine blue staining. C. Expression of collagen II, MMP3, and MMP13 detected by qPCR in normal human and OA chondrocytes. D and E. Cytotoxic effects of Xn on human chondrocytes determined at 24h and 48h by CCK8 assay. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: Primary antibodies specifically directed against GAPDH (1:1000), COX-2 (1:1000), iNOS (1:1000), MMP1 (1:5000), MMP3 (1:5000), MMP13 (1:1000), PGC-1α (1:1000), TFAM (1:1000), AMPKα (1:1000), and p-AMPKα (1:1000) were purchased from Proteintech.

Techniques: Staining, Expressing, CCK-8 Assay

Journal: Heliyon

Article Title: Xanthohumol alleviates palmitate-induced inflammation and prevents osteoarthritis progression by attenuating mitochondria dysfunction/NLRP3 inflammasome axis

doi: 10.1016/j.heliyon.2023.e21282

Figure Lengend Snippet: Attenuation of PA-induced ECM degradation by Xn in human chondrocytes. A. mRNA expression of collagen II, MMP1, MMP3, MMP13, and Adamts5 detected using qPCR. Protein expression of collagen II, MMP1, MMP3, MMP13, and Adamts5 detected using western blotting (B) and quantification results (C). D. Expression of collagen II and MMP13 detected by immunofluorescence. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: Primary antibodies specifically directed against GAPDH (1:1000), COX-2 (1:1000), iNOS (1:1000), MMP1 (1:5000), MMP3 (1:5000), MMP13 (1:1000), PGC-1α (1:1000), TFAM (1:1000), AMPKα (1:1000), and p-AMPKα (1:1000) were purchased from Proteintech.

Techniques: Expressing, Western Blot, Immunofluorescence